Prokka is a Perl command-line tool for rapid annotation of prokaryotic genomes (bacteria, archaea, and viruses). It takes a FASTA genome assembly as input and produces GFF3, GenBank, and other standard annotation formats by running a pipeline of external tools: Prodigal for CDS prediction, Aragorn/Barrnap/RNAmmer for tRNA/rRNA, Infernal/Rfam for ncRNA, BLAST+ and HMMER3 for functional annotation against curated databases (Swiss-Prot, HAMAP, ISfinder, NCBI AMR). The architecture is a monolithic Perl script with helper scripts for database building and maintenance. While widely used and highly cited, the repository is explicitly unmaintained by the original author (who recommends migration to Bakta), dependencies are dated, and many internal scripts lack automated testing. Bundled components like the SwissKnife Perl modules for UniProt parsing are self-contained and potentially reusable in other bioinformatics pipelines.