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Gffread is a comprehensive C++ command-line utility for processing GFF/GTF/BED/TLF genomic annotation files. It provides format conversion, filtering, FASTA sequence extraction (exons, CDS, protein translations), junction analysis, transcript clustering into loci, and tabular output generation. The tool handles complex genomic features including splice site validation, CDS translation with frame handling, and redundant transcript removal. Built with a modular design separating core logic (gff_utils) from main application logic (gffread), it demonstrates solid bioinformatics software engineering practices.
RATING:
MAINT: active
DRIFT: low
▸ 4 reusable components found
── end of results ──